PHYSICAL SCIENCES TUTORIALS
X40 Following the Electrons: Simulation for High-Resolution STEM and CBEDs
Mark P. Oxley, Oak Ridge National Laboratory
Mark Oxley is a research scientist in the Materials Science and Technology Division at Oak Ridge National Laboratory. His expertise is the simulation and quantification of scanning transmission electron microscopy images and spectroscopy. He is also working on the accurate simulation of 4D STEM data sets to be used as training sets for deep learning algorithms.
- Introduction to basic STEM simulation techniques and the requirement for convergence
- Simulation of electron energy loss spectroscopy for core and low loss excitations
- The importance of including the contribution of electrons that have undergone thermal diffuse scattering
- Convergent beam diffraction patterns: requirements for quantitative simulation
X41 Entrepreneurship in the Microscopy Community
Daniel Masiel, Integrated Dynamic Electron Solutions
Dan founded Integrated Dynamic Electron Solutions (IDES) fresh out of grad school. IDES allows researchers to illuminate nanoscale dynamics with its line of time-resolved imaging products spanning femtosecond to millisecond time scales.
- Instrumentation development and commercialization
- Practical steps to take when starting your own business
- Business start-up best practices
- Financing a scientific instrumentation company
X42 Efficient Phase Contrast Imaging via Electron Ptychography
Timothy J. Pennycook, Max Planck Institute for Solid State Research, Germany
Timothy Pennycook is a Scientist at the Max Planck Institute for Solid State Research. His research focuses on developing methods to extract the maximum information out of samples, including using dose efficient 4D STEM methods such as ptychography to see beam sensitive materials more clearly before they are destroyed. He programmed the first implementation of single side band ptychography which has now evolved into ptychoSTEM, a free and open source package for performing ptychography.
- Introduction to ptychography
- Hardware considerations; fast cameras
- Introduction to the free and open source ptychoSTEM package
- Processing the data and performing post collection aberration correction and optical sectioning
BIOLOGICAL SCIENCES TUTORIALS
X43 Expanding the Computational Toolbox for CryoEM
Alberto Bartesaghi, Duke University
Alberto Bartesaghi, PhD is currently an Associate Professor of Computer Science, Electrical Engineering and Biochemistry. He pushed the resolution of cryoEM protein structure determination during his tenure as a post-doctoral fellow and staff scientist at the National Cancer Institute in the Sriram Subramaniam lab.
- Robust strategies for particle picking and sorting
- Per-particle frame alignment for high-resolution cryoEM
- Data-driven approaches for optimal exposure weighting
- Unsupervised image sorting using Machine Learning algorithms
- Towards fully automated cryoEM workflows
X44 High-End Data Collection for Single-Particle Cryo-EM
Felix Weis, The European Molecular Biology Laboratory - Heidelburg, Germany
Felix Weis joined EMBL Heidelberg in April 2017. He is a cryo-EM support scientist taking care of the EMBL platform, providing help, training and support to internal users as well as to external visitors of the facility. His research interests cover many aspects of cryo-EM, including sample preparation, imaging and processing.
- Sample optimization
- Acquisition strategies
- Automation and throughput
- Alternatives for difficult samples
X45 Tips and Tricks for High-Pressure Freezing / Freeze Substitution
Martin Schauflinger, University of Missouri
REPLACEMENT: Christopher Buser, Oak Crest Institute of Science
Christopher Buser, PhD is currently a senior faculty member at Oak Crest Institute of Science in Electron & Atomic Force Microscopy. His principal research interests include application of high-resolution imaging for biomedical contract research, and development of preparation techniques for atomic force microscopy.
- Sample preparation for high-pressure freezing
- Sample loading into a high-pressure freezer
- Modifying freeze substitution solutions
- Quick freeze substitution