Technologists' Forum Sessions

X30 - Utilization of the National NIH funded Cryo-EM Centers: Transformative High Resolution Cryo-Electron Microscopy

CHAIRS:
Claudia Lopez, Oregon Health & Science University
Janice G. Pennington, University of Wisconsin-Madison

• Sample preparation "Do's & Don'ts"
• Best approaches for data collection>
• Direct Electron detectors: uses and preferences
• Data processing and handling
• Best practices in a national laboratory
• "Personalities" of different centers

X31 - Roundtable: Technical Careers in Microscopy - For the Love of Microscopy

CHAIRS:
Phoebe J. Doss, University of Texas Southwestern Medical Center
Janice G. Pennington, University of Wisconsin-Madison

A panel of technologists with diverse backgrounds will discuss their careers in microscopy. Learn how they found out about microscopy, why they chose it, and how their career has evolved through the years. The panel will include early and late career technologists from academia and industry. Learn how to become a Certified Electron Microscopy Technologist (CEMT) and what it can do for you to promote your career. Join in the conversation and share your story!

• Technologists from diverse backgrounds in microscopy will speak about their careers.
• How did they find out about microscopy as a career? Why did they choose that instead of all the other options available?
• How has their career developed through the years and what advice do they have for technologists new to the field?

X32 - Imaging Resin Embedded Samples for Serial Block Face Imaging or Array Tomography

CHAIRS:
Janice G. Pennington, University of Wisconsin-Madison
Phoebe J. Doss, University of Texas Southwestern Medical Center

• Array tomography, a technique for imaging serial sections for 3D reconstruction, will be compared with SBFSEM and FIB SEM
• Tips for resin embedding of samples for SEM imaging
• Tips for preparing serial sections for array tomography
• Techniques for correlative light and electron microscopy