Sunday Short Courses
- All Sunday Short Courses start at 8:30 AM and end by 5:00 PM.
- Mid-morning and mid-afternoon coffee breaks are provided.
- Breakfast and lunch are on your own.
- A Certificate of Attendance will be emailed to each participant.
X-10 High-Resolution Structure Determination by Cryo-EM: What Could Possibly Go Wrong?
Anchi Cheng, New York Structural Biology Center
Steve Ludtke, Baylor College of Medicine
- Specimen preparation and plunge-freezing choices and considerations
- Data collection - Camera and TEM parameters, TEM automation
- Initial image processing from raw data
- Single-particle reconstruction, choices and validation
- Structure modeling and results presentation
X-11 Super-Resolution Microscopy: Potential, Mechanics, Implementation, and Practicalities
Bryan Millis, Vanderbilt University
Simon Watkins, University of Pittsburgh
- What is "super-resolution microscopy" and do you need it?
- What are the various approaches available and how does each work?
- What are the strengths and weaknesses of each method?
- Practicalities of running super-resolution imaging in a multi-user facility
- How practical is live-cell super-resolution microscopy?
- Common pitfalls of super-resolution microscopy
X-12 Selecting and Optimizing Image Information in the SEM and VPSEM
Bradley Thiel, SUNY Polytechnic Institute
- Understanding the imaging options for materials and biological application-specific SEM use
- Determining specific conditions for the most-relevant sample imaging
- Modern electron detection systems and stage/column variables for SEM/VPSEM
- Tools for measurement and resolution determination
X-13 Modern Electron Crystallography for Materials Sciences and Biology
Sergei Rouvimov, University of Notre Dame
Roberto Reis, Northwestern University
Peter Moeck, Portland State University
- Recent developments in electron crystallography for nanomaterials including soft and biological materials
- Basics of scanning electron diffraction methods for microstructure analysis including bio-crystals
- Electron crystallography applications for structural biology, including protein crystals
- Cryo-electron crystallography, including single-particle cryo-EM
- New experimental and computer-simulation techniques to improve the speed and reliability of structure characterization
X-14 In Situ and Operando Approaches to TEM
Robert Sinclair, Stanford University
Peter Crozier (tentative), Arizona State University
This course will introduce the fundamental concepts for in situ electron microscopy, and will include:
- Hot stages
- Gas cells
- Liquid cells
- Biasing holders
- Magnetic field
- Light illumination
X-15 Data Analysis in Materials Science
Duncan Johnstone, University of Cambridge, United Kingdom
Katherine E. MacArthur, Forschungszentrum JÃ¼lich, Germany
Magnus Nord, University of Antwerp, Belgium
Eric Prestat, University of Manchester, United Kingdom
Joshua Taillon, National Institute of Standards and Technology
- Introduction to HyperSpy and related Python libraries for multi-dimensional image and spectra processing and analysis
- Machine learning
- Big data analysis strategies
- Curve fitting of multi-dimensional datasets
- EELS and EDS analysis
- Atomic resolution image analysis